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Protocols by peptide

Current research protocols

A reference summary of dosing cadence, cycle length, and common stacking pairs drawn from the published research on popular peptides. Provided for informational purposes only — not medical advice, not for human consumption.

Tirzepatide

GLP-1 / GIP

Dual GIP and GLP-1 receptor agonist studied for metabolic and body-composition endpoints.

Typical dose
2.5 mg → titrated to 5–15 mg
Cadence
Once weekly
Cycle
Titrate every 4 weeks; 12–72 week studies
Route
Subcutaneous

Notes: Titration is used to mitigate GI side effects. Do not exceed 15 mg/week in study protocols without clinical oversight.

Retatrutide

GLP-1 / GIP / Glucagon

Triple agonist under investigation for weight-loss and hepatic endpoints; steeper titration than tirzepatide.

Typical dose
2 mg → titrated to 4–12 mg
Cadence
Once weekly
Cycle
48 weeks in Phase II trials
Route
Subcutaneous

Notes: GI side effects are dose-dependent; slow titration is standard in current trials.

Semaglutide

GLP-1

Selective GLP-1 receptor agonist with the largest published clinical dataset among incretin peptides.

Typical dose
0.25 mg → titrated to 1–2.4 mg
Cadence
Once weekly
Cycle
Titrate every 4 weeks; long-term studies to 68+ weeks
Route
Subcutaneous

Notes: STEP trial protocols escalate by 0.25 mg increments monthly.

BPC-157

Regenerative

Body Protection Compound derived from a gastric peptide; studied for tendon, ligament, and GI models.

Typical dose
250–500 mcg
Cadence
1–2× daily
Cycle
4–8 weeks on / 4 weeks off
Route
Subcutaneous (local to injury site preferred in animal studies)

Notes: Frequently paired with TB-500 in regenerative research stacks.

Commonly stacked with: TB-500, GHK-Cu

TB-500 (Thymosin β-4)

Regenerative

Actin-binding peptide investigated for cardiac, tendon, and wound-healing models.

Typical dose
2–5 mg loading; 2 mg maintenance
Cadence
2× weekly loading × 4 weeks; then 1× weekly
Cycle
6–8 weeks
Route
Subcutaneous or intramuscular

Notes: Loading phase is protocol-typical; drop to maintenance after 4 weeks.

Commonly stacked with: BPC-157

Ipamorelin

Growth Hormone Secretagogue

Selective GHRP with minimal effect on cortisol/prolactin in study models.

Typical dose
200–300 mcg
Cadence
2–3× daily (AM, pre/post-workout, pre-bed)
Cycle
8–12 weeks on / 4 weeks off
Route
Subcutaneous

Notes: Often paired 1:1 with CJC-1295 (no DAC) for pulsatile GH release studies.

Commonly stacked with: CJC-1295 (no DAC)

CJC-1295 (no DAC)

GHRH Analog

Short-half-life GHRH analog (Mod GRF 1-29) used to amplify GH pulses when paired with a GHRP.

Typical dose
100 mcg
Cadence
2–3× daily with a GHRP
Cycle
8–12 weeks on / 4 weeks off
Route
Subcutaneous

Notes: Without DAC, the compound has a short half-life (~30 min) — pairs stoichiometrically with ipamorelin at each pulse.

Commonly stacked with: Ipamorelin

MOTS-c

Mitochondrial

Mitochondrial-derived peptide studied for metabolic and exercise-adaptation endpoints.

Typical dose
5–10 mg
Cadence
2–3× per week
Cycle
4–8 weeks
Route
Subcutaneous

Notes: Studies commonly cluster injections on non-consecutive days.

AOD-9604

Fat-Loss Fragment

hGH C-terminal fragment (176-191) studied for lipolytic activity without GH/IGF-1 effects.

Typical dose
300 mcg
Cadence
Once daily, AM fasted
Cycle
8–12 weeks
Route
Subcutaneous

Notes: Fasted-state administration is standard in the published protocols.

GHK-Cu

Regenerative / Skin

Copper tripeptide studied for wound-healing, dermal remodeling, and hair-follicle endpoints.

Typical dose
1–2 mg (SC); topical variants common in skin studies
Cadence
Daily
Cycle
8–12 weeks
Route
Subcutaneous or topical

Notes: Do not co-administer in the same syringe as reducing agents.

SS-31 (Elamipretide)

Mitochondrial

Cardiolipin-targeted peptide investigated for mitochondrial function in cardiac and neurologic models.

Typical dose
1–5 mg
Cadence
Daily
Cycle
4–12 weeks
Route
Subcutaneous

Notes: Reconstituted volumes are small; use a low-volume syringe for accuracy.

Cagrilintide

Amylin Analog

Long-acting amylin analog investigated alongside GLP-1 agonists for appetite/weight endpoints.

Typical dose
0.3 mg → titrated to 2.4 mg
Cadence
Once weekly
Cycle
20+ weeks in trial protocols
Route
Subcutaneous

Notes: Frequently studied in combination with semaglutide (CagriSema).

Commonly stacked with: Semaglutide

Semax

Nootropic

ACTH(4-10) analog studied for cognitive and neuroprotective endpoints.

Typical dose
300–600 mcg per nostril
Cadence
1–3× daily
Cycle
2–4 weeks
Route
Intranasal

Notes: Split total daily dose across both nostrils; short cycles are protocol-typical.

Selank

Nootropic / Anxiolytic

Tuftsin analog investigated for anxiolytic and immunomodulatory endpoints.

Typical dose
250–500 mcg per nostril
Cadence
1–3× daily
Cycle
2–4 weeks
Route
Intranasal

Notes: Often alternated with Semax in nootropic research stacks.

Commonly stacked with: Semax

KLOW

Regenerative Blend

Blend of Kisspeptin, TB-500-like fragment, GHK-Cu, and BPC-157 studied for combined regenerative endpoints.

Typical dose
1–2 mg blend equivalent
Cadence
Daily to 5× per week
Cycle
4–8 weeks
Route
Subcutaneous

Notes: Because it is a blend, dose the total volume — each component's mg is set by the blend ratio.

NAD+

Coenzyme

Nicotinamide adenine dinucleotide studied for cellular energetics and longevity models.

Typical dose
50–100 mg
Cadence
Daily loading × 7–14 days; then 2–3× weekly
Cycle
4–12 weeks
Route
Subcutaneous or intramuscular

Notes: Flushing and injection-site discomfort are dose-dependent; slow-push technique is standard.

All protocols are summarized from publicly available research literature. Compounds sold by PepThrive Maevin are for in-vitro laboratory research only. Nothing on this page constitutes medical advice.